Cell-mediated cytotoxicity

Regulation of Cell-mediated Cytotoxicity

Mice were treated with sublethal and midlethal doses of irradiation (500-700 rads) and injected intravenously with allogeneic or semiallogeneic F(1) hybrid spleen cells. The cytotoxicity developed by their spleen cells was measured with the lysis of (51)Cr-labeled target cells and was found to be stronger (although delayed in time) than the cytotoxic activity of spleen cells from nonirradiated ...

Peroxidase-mediated Mammalian Cell Cytotoxicity

Lactoperoxidase, in the presence of hydrogen peroxide and iodide is cytotoxic for human and mouse lymphoid cells, and human erythrocytes. Myeloperoxidase, in amounts equivalent to 1.5 x 10(6) neutrophils, readily replaces lactoperoxidase, and allows the substitution of the iodide ion by chloride. The myeloperoxidase-mediated reaction is rapid, and highly efficient, leading to 85-90% cell death ...

Using impedance-based approaches for measuring cell-mediated cytotoxicity and antibody-dependent cell-mediated cytotoxicity (ADCC)

The most common method for measuring cell-mediated cytotoxicity is the release assay. Effector cell-mediated disruption of the target cell membrane results in leakage of its cytoplasmic contents into the culture medium. Endogenous biomolecules (such as lactate dehydrogenase) or previously added exogenous labels (such as the radioisotopes Cr or In) that leak into the media are then measured as a...

Molecular Mechanisms in Cell-Mediated Cytotoxicity

terleukin-1b converting enzyme (ICE) family, now called Department of Biomedical Science the caspase family (Alnemri et al., 1996), is believed to and Biotechnologies be required for cell death, at least as to its nuclear University of Brescia manifestations. GraB can cleave and thus activate cas25100 Brescia pases, leading tocell death. This pathway was examined and Institute of Histology by C...

Regulation of NK Cell-Mediated Cytotoxicity